Hotspot

atpI-atpH

LSC · 32052-32654 · 603 bp

Back to Melica

Pi 0.00535
MarkerSeek score 64.50
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
603
variable_sites
38
pi
0.00535
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TTTTGCAACATTAGCCGCAG GCAATAACAGAAGCAGCAGC 45.0 50.0 57.9 58.1 549-697 bp 1.0 87.2
atpI-atpH_p2 TTTTGCAACATTAGCCGCAG GCAGCAATAACAGAAGCAGC 45.0 50.0 57.9 58.1 552-700 bp 1.0 87.1
atpI-atpH_p3 TTTTGCAACATTAGCCGCAG AGCCAATCCAGCAGCAATAA 45.0 45.0 57.9 57.9 562-710 bp 1.0 86.8
atpI-atpH_p4 TTTTGCAACATTAGCCGCAG AAGCAGCAGCAATTAGTGGA 45.0 45.0 57.9 57.8 539-687 bp 1.0 86.3
atpI-atpH_p5 TTTTGCAACATTAGCCGCAG GCAGTACCTTGACCAACTCC 45.0 55.0 57.9 58.2 606-754 bp 1.0 86.2