Hotspot

trnC-rpoB

LSC · 18965-20070 · 1106 bp

Back to Melica

Pi 0.00564
MarkerSeek score 68.10
Primer available yes
Label trnC-rpoB

Feature Metadata

label_name
trnC-rpoB
feature_type
igs
region
LSC
length_bp
1106
variable_sites
90
pi
0.00564
unique_haplotype_count
50
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-rpoB_p1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1016-1200 bp 1.0 87.0
trnC-rpoB_p2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1015-1199 bp 1.0 87.0
trnC-rpoB_p3 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGCCTT 50.0 45.0 58.1 57.6 1080-1264 bp 1.0 86.4
trnC-rpoB_p4 AAAGGATTTGCAGTCCCCTG AGTTCTTCTGCCAAGCCTTG 50.0 50.0 58.1 58.4 1079-1263 bp 1.0 86.3
trnC-rpoB_p5 AAAGGATTTGCAGTCCCCTG CAAGTTCTTCTGCCAAGCCT 50.0 50.0 58.1 58.4 1081-1265 bp 1.0 86.2