Hotspot

trnD-psbM

LSC · 16391-17083 · 693 bp

Back to Melica

Pi 0.00656
MarkerSeek score 69.40
Primer available yes
Label trnD-psbM

Feature Metadata

label_name
trnD-psbM
feature_type
igs
region
LSC
length_bp
693
variable_sites
59
pi
0.00656
unique_haplotype_count
47
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-psbM_p1 TTCAATTGGTCAGAGCACCG AGAATGAACAGTGCAGTAGC 50.0 45.0 58.5 55.8 736-829 bp 1.0 68.3
trnD-psbM_p2 TAGGTATGCCATACACCCCG AGAATGAACAGTGCAGTAGC 55.0 45.0 58.7 55.8 768-861 bp 0.983333 65.8
trnD-psbM_p3 TTCAATTGGTCAGAGCACCG AGGAACTAGAATGAACAGTGCA 50.0 40.9 58.5 57.4 743-836 bp 1.0 65.0
trnD-psbM_p4 GTTCAATTGGTCAGAGCACC AGAATGAACAGTGCAGTAGC 50.0 45.0 57.0 55.8 737-830 bp 1.0 64.1
trnD-psbM_p5 TAGGTATGCCATACACCCCG AGGAACTAGAATGAACAGTGCA 55.0 40.9 58.7 57.4 775-868 bp 0.983333 62.6