Hotspot

trnG-trnT

LSC · 13605-15177 · 1573 bp

Back to Melica

Pi 0.00977
MarkerSeek score 79.10
Primer available yes
Label trnG-trnT

Feature Metadata

label_name
trnG-trnT
feature_type
igs
region
LSC
length_bp
1573
variable_sites
167
pi
0.00977
unique_haplotype_count
57
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-trnT_p1 ACGAATCACACTTTTACCACT TACGTCTTACCACGGCAGTA 38.1 50.0 55.5 58.2 1362-1738 bp 0.983333 61.3
trnG-trnT_p2 ACGAATCACACTTTTACCACT TACCACGGCAGTACTCTACC 38.1 55.0 55.5 58.2 1355-1731 bp 0.983333 61.1
trnG-trnT_p3 GAACGAATCACACTTTTACCACT TACGTCTTACCACGGCAGTA 39.1 50.0 57.3 58.2 1364-1740 bp 0.983333 60.3
trnG-trnT_p4 ACGAATCACACTTTTACCACT TCTTACCACGGCAGTACTCT 38.1 50.0 55.5 57.8 1358-1734 bp 0.933333 59.4
trnG-trnT_p5 ACGAATCACACTTTTACCACT GTCTTACCACGGCAGTACTC 38.1 55.0 55.5 57.7 1359-1735 bp 0.933333 58.8