Hotspot

trnG-trnfM

LSC · 12226-12691 · 466 bp

Back to Melica

Pi 0.0112
MarkerSeek score 67.40
Primer available yes
Label trnG-trnfM

Feature Metadata

label_name
trnG-trnfM
feature_type
igs
region
LSC
length_bp
466
variable_sites
55
pi
0.0112
unique_haplotype_count
42
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-trnfM_p1 GCGAGCGTAGTTCAATGGTA ACCAGTTCCCGGTATACTCC 50.0 55.0 58.1 58.2 527-658 bp 0.983333 88.1
trnG-trnfM_p2 GCGAGCGTAGTTCAATGGTA CCAGTTCCCGGTATACTCCA 50.0 55.0 58.1 58.2 526-657 bp 0.983333 88.1
trnG-trnfM_p3 GTCGACCCAAGCGGATATAC CGAGGCTCATAACCTTGAGG 55.0 55.0 57.9 57.8 518-649 bp 0.966667 87.7
trnG-trnfM_p4 GTCGACCCAAGCGGATATAC ACCAGTTCCCGGTATACTCC 55.0 55.0 57.9 58.2 567-698 bp 0.95 86.5
trnG-trnfM_p5 GTCGACCCAAGCGGATATAC CCAGTTCCCGGTATACTCCA 55.0 55.0 57.9 58.2 566-697 bp 0.95 86.5