Hotspot

trnS-psbD

LSC · 7868-8820 · 953 bp

Pi 0.00592

MarkerSeek score 70.20

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 74
pi: 0.00592
unique_haplotype_count: 47
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	GACAAAAACGAAACGGTCCC	45.0	50.0	58.0	57.9	1074-1156 bp	1.0	88.3
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	45.0	55.0	58.0	58.1	1095-1177 bp	1.0	88.2
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1073-1155 bp	1.0	87.2
trnS-psbD_p4	ATTAGCAATCCGCCGCTTTA	CCGACAAAAACGAAACGGTC	45.0	50.0	58.0	58.3	1076-1158 bp	1.0	87.0
trnS-psbD_p5	ATTAGCAATCCGCCGCTTTA	CGACAAAAACGAAACGGTCC	45.0	50.0	58.0	58.3	1075-1157 bp	1.0	87.0