Hotspot

rps16-trnQ

LSC · 5554-6541 · 988 bp

Back to Melica

Pi 0.00831
MarkerSeek score 62.20
Primer available yes
Label rps16-trnQ

Feature Metadata

label_name
rps16-trnQ
feature_type
igs
region
LSC
length_bp
988
variable_sites
102
pi
0.00831
unique_haplotype_count
50
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_p1 AAGTCGCACGTTGCTTTCTA AGCAGCTTGCCAAACAAAAG 45.0 45.0 58.1 58.1 1390-1649 bp 1.0 88.7
rps16-trnQ_p2 AAGTCGCACGTTGCTTTCTA CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.1 57.9 1395-1654 bp 1.0 88.6
rps16-trnQ_p3 GACTATGTCGAGCCAAGAGC AGCAGCTTGCCAAACAAAAG 55.0 45.0 58.2 58.1 1467-1726 bp 1.0 88.3
rps16-trnQ_p4 GACTATGTCGAGCCAAGAGC CTTACAGCAGCTTGCCAAAC 55.0 50.0 58.2 57.9 1472-1731 bp 1.0 88.3
rps16-trnQ_p5 TCATGTCCTTCAAGTCGCAC AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.2 58.1 1401-1660 bp 1.0 88.1