Hotspot

ndhI-ndhA

SSC · 124728-124809 · 82 bp

Back to Melia

Pi 0.0494
MarkerSeek score 70.40
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
82
variable_sites
4
pi
0.0494
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 ACCTTGCAGCTCGTATTGTT TTCCCAACTTCTTTCGCTGT 45.0 45.0 57.8 57.7 164-165 bp 1.0 78.2
ndhI-ndhA_p2 TTTGCCCAATGTACCTTGCA TTCCCAACTTCTTTCGCTGT 45.0 45.0 58.3 57.7 176-177 bp 1.0 77.2
ndhI-ndhA_p3 CTTTGCCCAATGTACCTTGC TTCCCAACTTCTTTCGCTGT 50.0 45.0 57.6 57.7 177-178 bp 1.0 76.6
ndhI-ndhA_p4 CAATGTACCTTGCAGCTCGT TTCCCAACTTCTTTCGCTGT 50.0 45.0 58.6 57.7 170-171 bp 1.0 75.2
ndhI-ndhA_p5 TTGCCCAATGTACCTTGCAG TTCCCAACTTCTTTCGCTGT 50.0 45.0 58.7 57.7 175-176 bp 1.0 73.6