Hotspot

rps11-rpl36

LSC · 83339-83459 · 121 bp

Back to Melia

Pi 0.0342
MarkerSeek score 72.30
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
121
variable_sites
4
pi
0.0342
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 CGTGCACTTTTACGTGAACC AAAATAAGGGCCTCGGTTCG 50.0 50.0 58.0 58.3 300-308 bp 1.0 80.0
rps11-rpl36_p2 AACCAATCCGTCCAGTCCTA AAAATAAGGGCCTCGGTTCG 50.0 50.0 58.1 58.3 284-292 bp 1.0 79.8
rps11-rpl36_p3 TTACGTGAACCAATCCGTCC AAAATAAGGGCCTCGGTTCG 50.0 50.0 57.9 58.3 291-299 bp 1.0 79.5
rps11-rpl36_p4 CGTGCACTTTTACGTGAACC AATAAGGGCCTCGGTTCGTA 50.0 50.0 58.0 58.5 298-306 bp 1.0 77.9
rps11-rpl36_p5 CGTGCACTTTTACGTGAACC ATAAGGGCCTCGGTTCGTAA 50.0 50.0 58.0 58.5 297-305 bp 1.0 77.9