Hotspot

petD-rpoA

LSC · 81614-81873 · 260 bp

Back to Melia

Pi 0.0231
MarkerSeek score 65.80
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
260
variable_sites
6
pi
0.0231
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 CCTTGTGCTTGGGTATTGGA TCGCATAGAAGATGTAAAACGGA 50.0 39.1 57.8 58.0 374-399 bp 1.0 55.5
petD-rpoA_p2 CAGTAGCGACAACCGTCTTT TCGCATAGAAGATGTAAAACGGA 50.0 39.1 58.2 58.0 413-438 bp 1.0 55.4
petD-rpoA_p3 CCTTGTGCTTGGGTATTGGA AGATGTAAAACGGATAGTGGGT 50.0 40.9 57.8 56.9 365-390 bp 1.0 54.7
petD-rpoA_p4 CAGTAGCGACAACCGTCTTT AGATGTAAAACGGATAGTGGGT 50.0 40.9 58.2 56.9 404-429 bp 1.0 54.5
petD-rpoA_p5 AGCGACAACCGTCTTTTTGA TCGCATAGAAGATGTAAAACGGA 45.0 39.1 58.3 58.0 409-434 bp 1.0 54.4