Hotspot

petA-psbJ

LSC · 66811-67304 · 494 bp

Back to Melia

Pi 0.0173
MarkerSeek score 70.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
494
variable_sites
8
pi
0.0173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CCATTGCGTGTTCAAGTCCT CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.8 58.1 634-943 bp 1.0 77.4
petA-psbJ_p2 TCCATTGCGTGTTCAAGTCC CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.8 58.1 635-944 bp 1.0 77.3
petA-psbJ_p3 CCATTGCGTGTTCAAGTCCT TCCGGATTAGGTTCGTCCTT 50.0 50.0 58.8 58.1 635-944 bp 1.0 76.7
petA-psbJ_p4 TGCGTGTTCAAGTCCTTTTG CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.2 58.1 630-939 bp 1.0 76.7
petA-psbJ_p5 GCGTGTTCAAGTCCTTTTGT CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.2 58.1 629-938 bp 1.0 76.7