Hotspot

rbcL-accD

LSC · 60121-60710 · 590 bp

Back to Melia

Pi 0.0191
MarkerSeek score 67.30
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
590
variable_sites
11
pi
0.0191
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTAGCAAATGGAGTCC TTTACTTAACCCGCACCTGT 55.0 45.0 58.0 57.1 755-766 bp 1.0 74.8
rbcL-accD_p2 TGCTCGCGAAGGTAATGAAA TTTACTTAACCCGCACCTGT 45.0 45.0 57.9 57.1 783-794 bp 1.0 74.4
rbcL-accD_p3 TTGCTCGCGAAGGTAATGAA TTTACTTAACCCGCACCTGT 45.0 45.0 57.9 57.1 784-795 bp 1.0 74.4
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC ACTTAACCCGCACCTGTATTC 55.0 47.6 58.0 58.0 752-763 bp 1.0 74.2
rbcL-accD_p5 TGCTCGCGAAGGTAATGAAA ACTTAACCCGCACCTGTATTC 45.0 47.6 57.9 58.0 780-791 bp 1.0 73.7