Hotspot

rps4-trnT-UGU

LSC · 49369-49718 · 350 bp

Back to Melia

Pi 0.026
MarkerSeek score 54.90
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
350
variable_sites
9
pi
0.026
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAAGATCGCTTCCGGCTCTA TTTAAGCCCGCTTAGCTCAG 50.0 50.0 58.0 58.0 525-531 bp 1.0 81.7
rps4-trnT-UGU_p2 AATTCCTCAGGGTGGGATGA TTTAAGCCCGCTTAGCTCAG 50.0 50.0 58.0 58.0 907-913 bp 1.0 81.1
rps4-trnT-UGU_p3 ATTCCTCAGGGTGGGATGAA TTTAAGCCCGCTTAGCTCAG 50.0 50.0 58.0 58.0 906-912 bp 1.0 81.1
rps4-trnT-UGU_p4 TTCCTCAGGGTGGGATGAAT TTTAAGCCCGCTTAGCTCAG 50.0 50.0 58.0 58.0 905-911 bp 1.0 81.1
rps4-trnT-UGU_p5 CAATTCCTCAGGGTGGGATG TTTAAGCCCGCTTAGCTCAG 55.0 50.0 57.9 58.0 908 bp 0.5 59.3