Hotspot

trnT-GGU-psbD

LSC · 34326-35577 · 1252 bp

Back to Melia

Pi 0.0185
MarkerSeek score 63.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1252
variable_sites
23
pi
0.0185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AAGCAATAGACCGGACCAAC 55.0 50.0 58.1 57.9 1424-1434 bp 1.0 80.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA AAGCAATAGACCGGACCAAC 50.0 50.0 58.1 57.9 1427-1437 bp 1.0 80.2
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1402-1412 bp 1.0 79.3
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1416-1426 bp 1.0 79.2
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1405-1415 bp 1.0 79.2