Hotspot

trnE-UUC-trnT-GGU

LSC · 33431-34253 · 823 bp

Back to Melia

Pi 0.0147
MarkerSeek score 65.40
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
823
variable_sites
12
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 903-910 bp 1.0 80.5
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 901-908 bp 1.0 80.5
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 957-964 bp 1.0 80.3
trnE-UUC-trnT-GGU_p4 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 882-889 bp 1.0 80.0
trnE-UUC-trnT-GGU_p5 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 880-887 bp 1.0 80.0