Hotspot

petN-psbM

LSC · 30735-32053 · 1319 bp

Back to Melia

Pi 0.0147
MarkerSeek score 65.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1319
variable_sites
16
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CTTGGGCCGCTTTAATGGTA TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1299-1469 bp 1.0 71.7
petN-psbM_p2 TTGGGCCGCTTTAATGGTAG TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1298-1468 bp 1.0 71.7
petN-psbM_p3 GGCCGCTTTAATGGTAGTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.7 57.6 1295-1465 bp 1.0 71.1
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1250-1420 bp 1.0 70.9
petN-psbM_p5 GGGCCGCTTTAATGGTAGTC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.7 57.6 1296-1466 bp 1.0 68.1