Hotspot

ycf3

LSC · 44085-46147 · 2063 bp

Back to Melastoma

Pi 0.00271

MarkerSeek score 50.80

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2063
variable_sites: 15
pi: 0.00271
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TCCCTTTTATTTGACATTTGACT	TCGTGGTGTCCAATTCCTTT	30.4	45.0	54.4	57.4	2199-2211 bp	1.0	40.6
ycf3_p2	TCCCTTTTATTTGACATTTGACT	TTCGTGGTGTCCAATTCCTT	30.4	45.0	54.4	57.4	2200-2212 bp	1.0	40.6
ycf3_p3	TCCCTTTTATTTGACATTTGACT	TTTCGTGGTGTCCAATTCCT	30.4	45.0	54.4	57.4	2201-2213 bp	1.0	40.6
ycf3_p4	TCCCTTTTATTTGACATTTGACT	CGTGGTGTCCAATTCCTTTG	30.4	50.0	54.4	57.3	2198-2210 bp	1.0	40.6
ycf3_p5	AGATCCCTTTTATTTGACATTTGACT	TCGTGGTGTCCAATTCCTTT	30.8	45.0	57.3	57.4	2202-2214 bp	1.0	40.6