Hotspot

rpl16

LSC · 83014-84457 · 1444 bp

Back to Melastoma

Pi 0.00369

MarkerSeek score 38.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1444
variable_sites: 16
pi: 0.00369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTTTTCCGTTGGTTCCACCT	ACCAACAAACACTTTTCTTTCAGT	45.0	33.3	57.9	57.9	1581-1592 bp	1.0	46.9
rpl16_p2	TTTTCCGTTGGTTCCACCTG	ACCAACAAACACTTTTCTTTCAGT	50.0	33.3	58.6	57.9	1580-1591 bp	1.0	43.2
rpl16_p3	TTTTTCCGTTGGTTCCACCT	CCAACAAACACTTTTCTTTCAGTC	45.0	37.5	57.9	57.4	1580-1591 bp	1.0	42.6
rpl16_p4	TTTTTCCGTTGGTTCCACCT	ACCAACAAACACTTTTCTTTCAG	45.0	34.8	57.9	56.3	1581-1592 bp	1.0	42.2
rpl16_p5	TTTTTCCGTTGGTTCCACCT	CCAACAAACACTTTTCTTTCAGT	45.0	34.8	57.9	56.3	1580-1591 bp	1.0	42.2