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psbT-psbN

LSC · 76052-76128 · 77 bp

Back to Melastoma

Pi 0.0433

MarkerSeek score 66.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 77
variable_sites: 10
pi: 0.0433
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GCCGAAAGTTCCAACGAAAA	ATCCATTCGAAGAACACGGG	45.0	50.0	57.5	58.0	128 bp	1.0	82.2
psbT-psbN_p2	GCCGAAAGTTCCAACGAAAA	TTCGAAGAACACGGGGACTA	45.0	50.0	57.5	58.4	123 bp	1.0	79.6
psbT-psbN_p3	TTTTTCGAGAACCGCCGAA	ATCCATTCGAAGAACACGGG	47.4	50.0	58.0	58.0	141 bp	1.0	77.4
psbT-psbN_p4	GCCGAAAGTTCCAACGAAAAA	ATCCATTCGAAGAACACGGG	42.9	50.0	58.2	58.0	128 bp	1.0	76.5
psbT-psbN_p5	GCCGAAAGTTCCAACGAAAA	TCGAAGAACACGGGGACTAG	45.0	55.0	57.5	58.8	122 bp	1.0	76.1