Hotspot

rpl20-clpP

LSC · 70573-71643 · 1071 bp

Back to Melastoma

Pi 0.00174

MarkerSeek score 53.90

Primer available yes

Label rpl20-clpP

Feature Metadata

label_name: rpl20-clpP
feature_type: igs
region: LSC
length_bp: 1071
variable_sites: 5
pi: 0.00174
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-clpP_p1	CTCGAATGAGCTCCACGAAA	TTATGTCAGCAACGGAAGCC	50.0	50.0	58.0	58.6	1219 bp	1.0	75.9
rpl20-clpP_p2	TCGAATGAGCTCCACGAAAG	TTATGTCAGCAACGGAAGCC	50.0	50.0	58.0	58.6	1218 bp	1.0	75.9
rpl20-clpP_p3	CTCGAATGAGCTCCACGAAA	GCCCAAGCTCATGGAATTGT	50.0	50.0	58.0	58.8	1202 bp	1.0	73.8
rpl20-clpP_p4	TCGAATGAGCTCCACGAAAG	GCCCAAGCTCATGGAATTGT	50.0	50.0	58.0	58.8	1201 bp	1.0	73.8
rpl20-clpP_p5	TGTTCGACGTCTACGAGCTA	TTATGTCAGCAACGGAAGCC	50.0	50.0	58.3	58.6	1175 bp	1.0	73.6