Hotspot

psaJ-rpl33

LSC · 68632-69178 · 547 bp

Back to Melastoma

Pi 0.00402

MarkerSeek score 52.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 6
pi: 0.00402
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GTCTTTGGCAGGTCTGTTGA	TTACTCGGGCATCTTTTCCC	50.0	50.0	58.0	57.6	641-646 bp	1.0	77.7
psaJ-rpl33_p2	GTCTTTGGCAGGTCTGTTGA	TACTCGGGCATCTTTTCCCT	50.0	50.0	58.0	58.4	640-645 bp	1.0	77.6
psaJ-rpl33_p3	CTCTCTGGTTCGGGTCTTTG	TTACTCGGGCATCTTTTCCC	55.0	50.0	57.9	57.6	654-659 bp	1.0	77.2
psaJ-rpl33_p4	CTCTCTGGTTCGGGTCTTTG	TACTCGGGCATCTTTTCCCT	55.0	50.0	57.9	58.4	653-658 bp	1.0	77.2
psaJ-rpl33_p5	AACCTATCTTTCCGTGGCAC	TTACTCGGGCATCTTTTCCC	50.0	50.0	57.9	57.6	686-691 bp	1.0	77.0