Hotspot

petL-petG

LSC · 67247-67430 · 184 bp

Back to Melastoma

Pi 0.0217

MarkerSeek score 44.10

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 12
pi: 0.0217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	GAGTGGATGCTCAAACGGAT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	843-845 bp	1.0	81.1
petL-petG_p2	GAGTGGATGCTCAAACGGAT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	821-823 bp	1.0	80.9
petL-petG_p3	GAGTGGATGCTCAAACGGAT	CGACGCTAGTCTAGGCATTC	50.0	55.0	58.0	58.0	951-960 bp	1.0	80.8
petL-petG_p4	GAGTGGATGCTCAAACGGAT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	807-809 bp	1.0	80.5
petL-petG_p5	CTTCAACGAAGAGAAGGGGG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	57.9	58.0	947-949 bp	1.0	80.3