Hotspot

atpB-rbcL

LSC · 56328-57124 · 797 bp

Back to Melastoma

Pi 0.0105

MarkerSeek score 47.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 25
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACTCCAGGAACCGAAG	GCTGCCAAGGTATCAGTAGG	55.0	55.0	58.2	57.8	951-954 bp	1.0	78.7
atpB-rbcL_p2	ACTCCAGGAACCGAAGTAGT	GCTGCCAAGGTATCAGTAGG	50.0	55.0	57.7	57.8	947-950 bp	1.0	77.8
atpB-rbcL_p3	GAAACTCCAGGAACCGAAGT	GCTGCCAAGGTATCAGTAGG	50.0	55.0	57.5	57.8	950-953 bp	1.0	75.8
atpB-rbcL_p4	GGAAACTCCAGGAACCGAAG	AATGCTGCCAAGGTATCAGT	55.0	45.0	58.2	57.2	954-957 bp	1.0	74.1
atpB-rbcL_p5	TGGAAACTCCAGGAACCGAA	GCTGCCAAGGTATCAGTAGG	50.0	55.0	58.9	57.8	952-955 bp	1.0	73.3