Hotspot

trnD-GUC-trnY-GUA

LSC · 31244-31699 · 456 bp

Back to Melastoma

Pi 0.0038
MarkerSeek score 52.80
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
456
variable_sites
5
pi
0.0038
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 517-528 bp 1.0 77.8
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 516-527 bp 1.0 77.8
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 538-549 bp 1.0 76.7
trnD-GUC-trnY-GUA_p4 TTTATTCCGTCGGGACTGAC TGGCAATATGTCTACGCTGG 50.0 50.0 57.3 57.8 570-581 bp 1.0 76.6
trnD-GUC-trnY-GUA_p5 TTTATTCCGTCGGGACTGAC GGCAATATGTCTACGCTGGT 50.0 50.0 57.3 57.8 569-580 bp 1.0 76.5