Hotspot

atpF-atpH

LSC · 13462-14034 · 573 bp

Back to Melastoma

Pi 0.00338

MarkerSeek score 55.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 5
pi: 0.00338
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TAAACCCAAAACTCCCAGCC	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.0	57.5	687-695 bp	1.0	76.9
atpF-atpH_p2	GGCCCAAGGAAAGGAAAGAA	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.0	57.5	657-665 bp	1.0	76.9
atpF-atpH_p3	GGCCCAAGGAAAGGAAAGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	722-730 bp	1.0	75.7
atpF-atpH_p4	TAAACCCAAAACTCCCAGCC	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	752-760 bp	1.0	75.6
atpF-atpH_p5	TAAACCCAAAACTCCCAGCC	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	753-761 bp	1.0	75.5