Hotspot

orf110-rns

Genome · 204756-209523 · 4768 bp

Back to Melanthalia

Pi 0.437

MarkerSeek score 74.10

Primer available yes

Label orf110-rns

Feature Metadata

label_name: orf110-rns
feature_type: igs
region: Genome
length_bp: 4768
variable_sites: 1707
pi: 0.437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf110-rns_p1	GCTGTCACCGATTTATCCGT	AGCAGAAACTTGACGAGCAA	50.0	45.0	58.1	58.0	712 bp	0.5	59.1
orf110-rns_p2	TGCTGTCACCGATTTATCCG	AGCAGAAACTTGACGAGCAA	50.0	45.0	58.1	58.0	713 bp	0.5	59.1
orf110-rns_p3	AACCAACTGCGTCTCTTCAG	AGCAGAAACTTGACGAGCAA	50.0	45.0	58.1	58.0	621 bp	0.5	58.6
orf110-rns_p4	GCTGTCACCGATTTATCCGT	ACAGACTTTCGCTTGCATCA	50.0	45.0	58.1	58.1	567 bp	0.5	58.6
orf110-rns_p5	TGCTGTCACCGATTTATCCG	ACAGACTTTCGCTTGCATCA	50.0	45.0	58.1	58.1	568 bp	0.5	58.5