Hotspot

orf73-orf120

Genome · 201244-202042 · 799 bp

Back to Melanthalia

Pi 0.464

MarkerSeek score 77.30

Primer available yes

Label orf73-orf120

Feature Metadata

label_name: orf73-orf120
feature_type: igs
region: Genome
length_bp: 799
variable_sites: 268
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf73-orf120_p1	AGCAGGATGAAAAAGGTGCA	AAGCACCCAGAGACAAGTTG	45.0	50.0	58.0	58.0	1495 bp	0.5	59.8
orf73-orf120_p2	TGCAGCAGGATGAAAAAGGT	AAGCACCCAGAGACAAGTTG	45.0	50.0	58.0	58.0	1498 bp	0.5	59.8
orf73-orf120_p3	GCAGGATGAAAAAGGTGCAC	AAGCACCCAGAGACAAGTTG	50.0	50.0	57.9	58.0	1494 bp	0.5	59.2
orf73-orf120_p4	GAGAGTGAATTGCAGCAGGA	AAGCACCCAGAGACAAGTTG	50.0	50.0	57.9	58.0	1508 bp	0.5	58.9
orf73-orf120_p5	AGAGAGTGAATTGCAGCAGG	AAGCACCCAGAGACAAGTTG	50.0	50.0	57.9	58.0	1509 bp	0.5	58.9