Hotspot

ompR-orf88

Genome · 194352-199230 · 4879 bp

Back to Melanthalia

Pi 0.396

MarkerSeek score 72.10

Primer available yes

Label ompR-orf88

Feature Metadata

label_name: ompR-orf88
feature_type: igs
region: Genome
length_bp: 4879
variable_sites: 1462
pi: 0.396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ompR-orf88_p1	TGGCATTTGCGGCTTATAGT	CTGCAAGACCAAACAGGACT	45.0	50.0	57.9	58.0	692 bp	0.5	59.4
ompR-orf88_p2	TGGCATTTGCGGCTTATAGT	AATCACGTAGCCTAGCTTGC	45.0	50.0	57.9	58.1	538 bp	0.5	59.1
ompR-orf88_p3	TATGGGGCCATCTGAGTCAA	CTGCAAGACCAAACAGGACT	50.0	50.0	58.1	58.0	1016 bp	0.5	58.9
ompR-orf88_p4	GTTATAGGCGTGGCATTTGC	CTGCAAGACCAAACAGGACT	50.0	50.0	57.9	58.0	702 bp	0.5	58.8
ompR-orf88_p5	ATCTGTATGGGGCCATCTGA	CTGCAAGACCAAACAGGACT	50.0	50.0	57.9	58.0	1021 bp	0.5	58.8