Hotspot

syfB-rps1

Genome · 175761-176263 · 503 bp

Back to Melanthalia

Pi 0.103
MarkerSeek score 81.40
Primer available yes
Label syfB-rps1

Feature Metadata

label_name
syfB-rps1
feature_type
igs
region
Genome
length_bp
503
variable_sites
49
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
syfB-rps1_p1 AGATCAGCCAAATCGCCTTT AAAAGCCCTTGCATTCTCGA 45.0 45.0 57.9 58.1 1116 bp 0.5 58.2
syfB-rps1_p2 CCAAATCGCCTTTGTTTGCA AAAAGCCCTTGCATTCTCGA 45.0 45.0 58.1 58.1 1109 bp 0.5 58.2
syfB-rps1_p3 AGCCAAATCGCCTTTGTTTG AAAAGCCCTTGCATTCTCGA 45.0 45.0 57.8 58.1 1111 bp 0.5 58.1
syfB-rps1_p4 CAGCCAAATCGCCTTTGTTT AAAAGCCCTTGCATTCTCGA 45.0 45.0 57.8 58.1 1112 bp 0.5 58.1
syfB-rps1_p5 AGATCAGCCAAATCGCCTTT AAGCCCTTGCATTCTCGATT 45.0 45.0 57.9 57.9 1114 bp 0.5 57.8