Hotspot

rpl19-dnaB

Genome · 161414-162356 · 943 bp

Back to Melanthalia

Pi 0.287

MarkerSeek score 82.90

Primer available yes

Label rpl19-dnaB

Feature Metadata

label_name: rpl19-dnaB
feature_type: igs
region: Genome
length_bp: 943
variable_sites: 248
pi: 0.287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl19-dnaB_p1	CCAACGTAGGTAAGACCAGC	GATGATGCTTCAGCCTGACA	55.0	50.0	58.0	58.0	2130-2311 bp	1.0	91.0
rpl19-dnaB_p2	CCAACGTAGGTAAGACCAGC	TGATGATGCTTCAGCCTGAC	55.0	50.0	58.0	58.0	2131-2312 bp	1.0	91.0
rpl19-dnaB_p3	AGCCTTGTTGCTTTTCCTGA	GATGATGCTTCAGCCTGACA	45.0	50.0	57.9	58.0	1852-2044 bp	1.0	90.4
rpl19-dnaB_p4	AGCCTTGTTGCTTTTCCTGA	TGATGATGCTTCAGCCTGAC	45.0	50.0	57.9	58.0	1853-2045 bp	1.0	90.4
rpl19-dnaB_p5	CCAACGTAGGTAAGACCAGC	ACTGATGATGCTTCAGCCTG	55.0	50.0	58.0	58.0	2133 bp	0.5	59.6