Hotspot

rpl35-bas1

Genome · 119513-120446 · 934 bp

Back to Melanthalia

Pi 0.278

MarkerSeek score 83.20

Primer available yes

Label rpl35-bas1

Feature Metadata

label_name: rpl35-bas1
feature_type: igs
region: Genome
length_bp: 934
variable_sites: 251
pi: 0.278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl35-bas1_p1	TTTACGAGCAACGCTACCTC	TGGTGGCTTAGGTGACCTAA	50.0	50.0	58.0	58.0	1508 bp	0.5	59.6
rpl35-bas1_p2	TTTACGAGCAACGCTACCTC	AACACGGAAATTCTGGGCAT	50.0	45.0	58.0	58.1	1582 bp	0.5	59.3
rpl35-bas1_p3	TTTACGAGCAACGCTACCTC	AGTCTCATCCTGACGAGGTT	50.0	50.0	58.0	57.8	1293 bp	0.5	58.1
rpl35-bas1_p4	TTTACGAGCAACGCTACCTC	TCAGTCTCATCCTGACGAGG	50.0	55.0	58.0	58.2	1295 bp	0.5	57.9
rpl35-bas1_p5	TTTACGAGCAACGCTACCTC	CATCCTGACGAGGTTTGTCC	50.0	55.0	58.0	58.3	1288 bp	0.5	57.7