Hotspot

rps20-rpl33

Genome · 102903-103631 · 729 bp

Back to Melanthalia

Pi 0.263

MarkerSeek score 80.70

Primer available yes

Label rps20-rpl33

Feature Metadata

label_name: rps20-rpl33
feature_type: igs
region: Genome
length_bp: 729
variable_sites: 188
pi: 0.263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps20-rpl33_p1	CGACAATAACGGGAAAGGAGT	TGTTTTGCAGTAAGCCCTGT	47.6	45.0	58.0	57.9	1718 bp	0.5	51.2
rps20-rpl33_p2	CGACAATAACGGGAAAGGAGT	GTTGCTTCTGCTGTTTTGCA	47.6	45.0	58.0	58.1	1729 bp	0.5	51.2
rps20-rpl33_p3	TGACAGCCTCATGATTTAGCC	TGTTTTGCAGTAAGCCCTGT	47.6	45.0	58.1	57.9	1594 bp	0.5	50.9
rps20-rpl33_p4	CGACAATAACGGGAAAGGAGT	GTGGCCTGATGTGTGAAGAT	47.6	50.0	58.0	57.9	1966 bp	0.5	50.8
rps20-rpl33_p5	TGACAGCCTCATGATTTAGCC	GTTGCTTCTGCTGTTTTGCA	47.6	45.0	58.1	58.1	1605 bp	0.5	50.8