Hotspot

cemA-orf687

Genome · 78148-79552 · 1405 bp

Back to Melanthalia

Pi 0.263

MarkerSeek score 80.80

Primer available yes

Label cemA-orf687

Feature Metadata

label_name: cemA-orf687
feature_type: igs
region: Genome
length_bp: 1405
variable_sites: 312
pi: 0.263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-orf687_p1	GCACCGGAAAGAAAATCTGC	GGCAGGCCTATTTGGTTCTT	50.0	50.0	58.0	58.1	2369 bp	0.5	58.7
cemA-orf687_p2	GCACCGGAAAGAAAATCTGC	GTGTGTTGAATGCCATGCAA	50.0	45.0	58.0	57.8	2014 bp	0.5	58.6
cemA-orf687_p3	GCAGAAGAACTTTCGTTGGC	GGCAGGCCTATTTGGTTCTT	50.0	50.0	58.0	58.1	2333 bp	0.5	58.5
cemA-orf687_p4	GCAGAAGAACTTTCGTTGGC	GTGTGTTGAATGCCATGCAA	50.0	45.0	58.0	57.8	1978 bp	0.5	58.5
cemA-orf687_p5	CTAGTACAGGCACCACCAAC	GGCAGGCCTATTTGGTTCTT	55.0	50.0	57.9	58.1	2080 bp	0.5	58.1