Hotspot

ycf46-psaM

Genome · 27633-28280 · 648 bp

Back to Melanthalia

Pi 0.144

MarkerSeek score 80.30

Primer available yes

Label ycf46-psaM

Feature Metadata

label_name: ycf46-psaM
feature_type: igs
region: Genome
length_bp: 648
variable_sites: 93
pi: 0.144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46-psaM_p1	AAACGAATGCGACAGGCTAT	TACTGAAGGGGCTAAAGGCT	45.0	50.0	58.0	58.0	1295 bp	0.5	59.5
ycf46-psaM_p2	GTGGGCGAATCTGAAAAACG	TACTGAAGGGGCTAAAGGCT	50.0	50.0	58.0	58.0	1310 bp	0.5	59.4
ycf46-psaM_p3	TGGGCGAATCTGAAAAACGA	TACTGAAGGGGCTAAAGGCT	45.0	50.0	58.1	58.0	1309 bp	0.5	58.8
ycf46-psaM_p4	TTGTTGATGGGCATACAGGG	TACTGAAGGGGCTAAAGGCT	50.0	50.0	57.9	58.0	1421 bp	0.5	58.6
ycf46-psaM_p5	GGCATACAGGGAACGGAAAA	TACTGAAGGGGCTAAAGGCT	50.0	50.0	58.2	58.0	1412 bp	0.5	58.4