primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf46-psaM_p1	ycf46-psaM	1	AAACGAATGCGACAGGCTAT	TACTGAAGGGGCTAAAGGCT	20	20	45.0	50.0	58.0	58.0	0.0	3.4	0.057331	27633	28280	1295-1295	0.500000	59.5
ycf46-psaM_p2	ycf46-psaM	2	GTGGGCGAATCTGAAAAACG	TACTGAAGGGGCTAAAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	3.4	0.069408	27633	28280	1310-1310	0.500000	59.4
ycf46-psaM_p3	ycf46-psaM	3	TGGGCGAATCTGAAAAACGA	TACTGAAGGGGCTAAAGGCT	20	20	45.0	50.0	58.1	58.0	0.0	3.4	0.147595	27633	28280	1309-1309	0.500000	58.8
ycf46-psaM_p4	ycf46-psaM	4	TTGTTGATGGGCATACAGGG	TACTGAAGGGGCTAAAGGCT	20	20	50.0	50.0	57.9	58.0	0.0	3.4	0.175511	27633	28280	1421-1421	0.500000	58.6
ycf46-psaM_p5	ycf46-psaM	5	GGCATACAGGGAACGGAAAA	TACTGAAGGGGCTAAAGGCT	20	20	50.0	50.0	58.2	58.0	0.0	3.4	0.204176	27633	28280	1412-1412	0.500000	58.4
ycf21-cpcA_p1	ycf21-cpcA	1	TAGCCTCCATTCTGCAGGAA	GAGCATCAGTAGCAGTTGCT	20	20	50.0	50.0	58.4	58.0	15.1	11.8	0.437799	46096	47748	2458-2458	0.500000	56.5
ycf21-cpcA_p2	ycf21-cpcA	2	TTAGCCTCCATTCTGCAGGA	GAGCATCAGTAGCAGTTGCT	20	20	50.0	50.0	58.4	58.0	15.1	11.8	0.437799	46096	47748	2459-2459	0.500000	56.5
ycf21-cpcA_p3	ycf21-cpcA	3	TAGCCTCCATTCTGCAGGAA	CATGGACTAGGTGGACAAGC	20	20	50.0	55.0	58.4	58.0	15.1	0.0	0.446282	46096	47748	1836-1836	0.500000	56.4
ycf21-cpcA_p4	ycf21-cpcA	4	TTAGCCTCCATTCTGCAGGA	CATGGACTAGGTGGACAAGC	20	20	50.0	55.0	58.4	58.0	15.1	0.0	0.446282	46096	47748	1837-1837	0.500000	56.4
ycf21-cpcA_p5	ycf21-cpcA	5	TAGCCTCCATTCTGCAGGAA	ATGGACTAGGTGGACAAGCT	20	20	50.0	50.0	58.4	58.0	15.1	0.0	0.458156	46096	47748	1835-1835	0.500000	56.3
psaC-ycf22_p1	psaC-ycf22	1	AGCAGGTATGACAGGTGGAT	ATTCCAGCTCGAATATCGGC	20	20	50.0	50.0	58.1	57.9	0.0	4.6	0.201005	54544	55261	2037-2037	0.500000	58.4
psaC-ycf22_p2	psaC-ycf22	2	CCCCTGGGATGGTTGTAAAG	ATTCCAGCTCGAATATCGGC	20	20	55.0	50.0	57.9	57.9	16.2	4.6	0.229930	54544	55261	1450-1450	0.500000	58.2
psaC-ycf22_p3	psaC-ycf22	3	ACTAGTCGAAGCATGGGTCT	ATTCCAGCTCGAATATCGGC	20	20	50.0	50.0	58.1	57.9	0.0	4.6	0.232091	54544	55261	1320-1320	0.500000	58.1
psaC-ycf22_p4	psaC-ycf22	4	GGGATGGTTGTAAAGCTGCT	ATTCCAGCTCGAATATCGGC	20	20	50.0	50.0	58.2	57.9	0.0	4.6	0.246631	54544	55261	1445-1445	0.500000	58.0
psaC-ycf22_p5	psaC-ycf22	5	TGCTCCTAGAACCGAGGATT	ATTCCAGCTCGAATATCGGC	20	20	50.0	50.0	57.8	57.9	0.0	4.6	0.246775	54544	55261	1414-1414	0.500000	58.0
trnR(ccg)-orf460_p1	trnR(ccg)-orf460	1	GTTTCCTCCTGGCCGTAAAA	ACAATGGAAAAGGATGCCGA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.314131	63449	65038	2795-2795	0.500000	57.5
trnR(ccg)-orf460_p2	trnR(ccg)-orf460	2	GCCTAAAGGTTCAGTCAGCA	ACAATGGAAAAGGATGCCGA	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.399380	63449	65038	2497-2497	0.500000	56.8
trnR(ccg)-orf460_p3	trnR(ccg)-orf460	3	TGCCTAAAGGTTCAGTCAGC	ACAATGGAAAAGGATGCCGA	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.399380	63449	65038	2498-2498	0.500000	56.8
trnR(ccg)-orf460_p4	trnR(ccg)-orf460	4	TTTCCTCCTGGCCGTAAAAG	ACAATGGAAAAGGATGCCGA	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.408577	63449	65038	2794-2794	0.500000	56.7
trnR(ccg)-orf460_p5	trnR(ccg)-orf460	5	AGAGCGTGTAACTCAGAGGA	ACAATGGAAAAGGATGCCGA	20	20	50.0	45.0	57.8	57.8	1.5	0.0	0.416297	63449	65038	2178-2178	0.500000	56.7
cemA-orf687_p1	cemA-orf687	1	GCACCGGAAAGAAAATCTGC	GGCAGGCCTATTTGGTTCTT	20	20	50.0	50.0	58.0	58.1	9.5	4.0	0.164755	78148	79552	2369-2369	0.500000	58.7
cemA-orf687_p2	cemA-orf687	2	GCACCGGAAAGAAAATCTGC	GTGTGTTGAATGCCATGCAA	20	20	50.0	45.0	58.0	57.8	9.5	0.0	0.169269	78148	79552	2014-2014	0.500000	58.6
cemA-orf687_p3	cemA-orf687	3	GCAGAAGAACTTTCGTTGGC	GGCAGGCCTATTTGGTTCTT	20	20	50.0	50.0	58.0	58.1	0.0	4.0	0.185308	78148	79552	2333-2333	0.500000	58.5
cemA-orf687_p4	cemA-orf687	4	GCAGAAGAACTTTCGTTGGC	GTGTGTTGAATGCCATGCAA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.189822	78148	79552	1978-1978	0.500000	58.5
cemA-orf687_p5	cemA-orf687	5	CTAGTACAGGCACCACCAAC	GGCAGGCCTATTTGGTTCTT	20	20	55.0	50.0	57.9	58.1	0.0	4.0	0.242130	78148	79552	2080-2080	0.500000	58.1
rps20-rpl33_p1	rps20-rpl33	1	CGACAATAACGGGAAAGGAGT	TGTTTTGCAGTAAGCCCTGT	21	20	47.6	45.0	58.0	57.9	0.0	0.0	1.097223	102903	103631	1718-1718	0.500000	51.2
rps20-rpl33_p2	rps20-rpl33	2	CGACAATAACGGGAAAGGAGT	GTTGCTTCTGCTGTTTTGCA	21	20	47.6	45.0	58.0	58.1	0.0	0.0	1.106095	102903	103631	1729-1729	0.500000	51.2
rps20-rpl33_p3	rps20-rpl33	3	TGACAGCCTCATGATTTAGCC	TGTTTTGCAGTAAGCCCTGT	21	20	47.6	45.0	58.1	57.9	0.0	0.0	1.142778	102903	103631	1594-1594	0.500000	50.9
rps20-rpl33_p4	rps20-rpl33	4	CGACAATAACGGGAAAGGAGT	GTGGCCTGATGTGTGAAGAT	21	20	47.6	50.0	58.0	57.9	0.0	3.3	1.151126	102903	103631	1966-1966	0.500000	50.8
rps20-rpl33_p5	rps20-rpl33	5	TGACAGCCTCATGATTTAGCC	GTTGCTTCTGCTGTTTTGCA	21	20	47.6	45.0	58.1	58.1	0.0	0.0	1.151650	102903	103631	1605-1605	0.500000	50.8
rpl35-bas1_p1	rpl35-bas1	1	TTTACGAGCAACGCTACCTC	TGGTGGCTTAGGTGACCTAA	20	20	50.0	50.0	58.0	58.0	0.0	21.9	0.046267	119513	120446	1508-1508	0.500000	59.6
rpl35-bas1_p2	rpl35-bas1	2	TTTACGAGCAACGCTACCTC	AACACGGAAATTCTGGGCAT	20	20	50.0	45.0	58.0	58.1	0.0	3.6	0.089946	119513	120446	1582-1582	0.500000	59.3
rpl35-bas1_p3	rpl35-bas1	3	TTTACGAGCAACGCTACCTC	AGTCTCATCCTGACGAGGTT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.237105	119513	120446	1293-1293	0.500000	58.1
rpl35-bas1_p4	rpl35-bas1	4	TTTACGAGCAACGCTACCTC	TCAGTCTCATCCTGACGAGG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.256872	119513	120446	1295-1295	0.500000	57.9
rpl35-bas1_p5	rpl35-bas1	5	TTTACGAGCAACGCTACCTC	CATCCTGACGAGGTTTGTCC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.286449	119513	120446	1288-1288	0.500000	57.7
rpl19-dnaB_p1	rpl19-dnaB	1	CCAACGTAGGTAAGACCAGC	GATGATGCTTCAGCCTGACA	20	20	55.0	50.0	58.0	58.0	0.0	6.6	0.040075	161414	162356	2130-2311	1.000000	91.0
rpl19-dnaB_p2	rpl19-dnaB	2	CCAACGTAGGTAAGACCAGC	TGATGATGCTTCAGCCTGAC	20	20	55.0	50.0	58.0	58.0	0.0	14.5	0.040075	161414	162356	2131-2312	1.000000	91.0
rpl19-dnaB_p3	rpl19-dnaB	3	AGCCTTGTTGCTTTTCCTGA	GATGATGCTTCAGCCTGACA	20	20	45.0	50.0	57.9	58.0	0.0	6.6	0.105882	161414	162356	1852-2044	1.000000	90.4
rpl19-dnaB_p4	rpl19-dnaB	4	AGCCTTGTTGCTTTTCCTGA	TGATGATGCTTCAGCCTGAC	20	20	45.0	50.0	57.9	58.0	0.0	14.5	0.105882	161414	162356	1853-2045	1.000000	90.4
rpl19-dnaB_p5	rpl19-dnaB	5	CCAACGTAGGTAAGACCAGC	ACTGATGATGCTTCAGCCTG	20	20	55.0	50.0	58.0	58.0	0.0	14.5	0.045789	161414	162356	2133-2133	0.500000	59.6
clpC-psbW_p1	clpC-psbW	1	TGCGCAAAAAGTAGGTTCAG	GCTTGCAGGGCTTTCATGTA	20	20	45.0	50.0	56.9	58.8	8.2	0.0	1.880569	167533	167652	707-707	0.500000	45.0
clpC-psbW_p2	clpC-psbW	2	GCGCAAAAAGTAGGTTCAGT	GCTTGCAGGGCTTTCATGTA	20	20	45.0	50.0	56.9	58.8	0.3	0.0	1.882622	167533	167652	706-706	0.500000	44.9
clpC-psbW_p3	clpC-psbW	3	TGCGCAAAAAGTAGGTTCAG	GCACAGCTTAACACTACTGC	20	20	45.0	50.0	56.9	57.1	8.2	0.0	1.939838	167533	167652	850-850	0.500000	44.5
clpC-psbW_p4	clpC-psbW	4	GCGCAAAAAGTAGGTTCAGT	GCACAGCTTAACACTACTGC	20	20	45.0	50.0	56.9	57.1	0.3	0.0	1.941891	167533	167652	849-849	0.500000	44.5
clpC-psbW_p5	clpC-psbW	5	TTGCGCAAAAAGTAGGTTCAG	GCTTGCAGGGCTTTCATGTA	21	20	42.9	50.0	57.7	58.8	22.8	0.0	2.171431	167533	167652	708-708	0.500000	42.6
syfB-rps1_p1	syfB-rps1	1	AGATCAGCCAAATCGCCTTT	AAAAGCCCTTGCATTCTCGA	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.225089	175761	176263	1116-1116	0.500000	58.2
syfB-rps1_p2	syfB-rps1	2	CCAAATCGCCTTTGTTTGCA	AAAAGCCCTTGCATTCTCGA	20	20	45.0	45.0	58.1	58.1	8.8	0.0	0.227033	175761	176263	1109-1109	0.500000	58.2
syfB-rps1_p3	syfB-rps1	3	AGCCAAATCGCCTTTGTTTG	AAAAGCCCTTGCATTCTCGA	20	20	45.0	45.0	57.8	58.1	8.8	0.0	0.243667	175761	176263	1111-1111	0.500000	58.1
syfB-rps1_p4	syfB-rps1	4	CAGCCAAATCGCCTTTGTTT	AAAAGCCCTTGCATTCTCGA	20	20	45.0	45.0	57.8	58.1	8.8	0.0	0.243667	175761	176263	1112-1112	0.500000	58.1
syfB-rps1_p5	syfB-rps1	5	AGATCAGCCAAATCGCCTTT	AAGCCCTTGCATTCTCGATT	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.269578	175761	176263	1114-1114	0.500000	57.8
psbD-ompR_p1	psbD-ompR	1	TGGACCCCACAGCAGTAATA	GCAAGAAGTTTGGGGGTACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050584	192724	193622	1353-1353	0.500000	59.6
psbD-ompR_p2	psbD-ompR	2	TGGACCCCACAGCAGTAATA	TGCAAGAAGTTTGGGGGTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050584	192724	193622	1354-1354	0.500000	59.6
psbD-ompR_p3	psbD-ompR	3	CGCTAAGCCGTGAGTATACC	GCAAGAAGTTTGGGGGTACA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.057973	192724	193622	1260-1260	0.500000	59.5
psbD-ompR_p4	psbD-ompR	4	CGCTAAGCCGTGAGTATACC	TGCAAGAAGTTTGGGGGTAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.057973	192724	193622	1261-1261	0.500000	59.5
psbD-ompR_p5	psbD-ompR	5	CCCATGCTATTTGCTGGAGT	GCAAGAAGTTTGGGGGTACA	20	20	50.0	50.0	57.9	58.0	9.1	0.0	0.073264	192724	193622	1325-1325	0.500000	59.4
ompR-orf88_p1	ompR-orf88	1	TGGCATTTGCGGCTTATAGT	CTGCAAGACCAAACAGGACT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.078462	196865	196984	692-692	0.500000	59.4
ompR-orf88_p2	ompR-orf88	2	TGGCATTTGCGGCTTATAGT	AATCACGTAGCCTAGCTTGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.110009	196865	196984	538-538	0.500000	59.1
ompR-orf88_p3	ompR-orf88	3	TATGGGGCCATCTGAGTCAA	CTGCAAGACCAAACAGGACT	20	20	50.0	50.0	58.1	58.0	4.4	0.0	0.135195	196865	196984	1016-1016	0.500000	58.9
ompR-orf88_p4	ompR-orf88	4	GTTATAGGCGTGGCATTTGC	CTGCAAGACCAAACAGGACT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.146799	196865	196984	702-702	0.500000	58.8
ompR-orf88_p5	ompR-orf88	5	ATCTGTATGGGGCCATCTGA	CTGCAAGACCAAACAGGACT	20	20	50.0	50.0	57.9	58.0	4.4	0.0	0.150146	196865	196984	1021-1021	0.500000	58.8
orf88-orf89_p1	orf88-orf89	1	ATAGCATCCAGAGGCTCGTA	ACCTTTTTCATCCTGCTGCA	20	20	50.0	45.0	57.7	58.0	10.5	0.9	0.294801	199498	200737	1730-1730	0.500000	57.6
orf88-orf89_p2	orf88-orf89	2	ATAGCATCCAGAGGCTCGTA	TGCACCTTTTTCATCCTGCT	20	20	50.0	45.0	57.7	58.0	10.5	0.0	0.294801	199498	200737	1733-1733	0.500000	57.6
orf88-orf89_p3	orf88-orf89	3	ACGTCTTTCCGTTTGGACTT	ACCTTTTTCATCCTGCTGCA	20	20	45.0	45.0	57.7	58.0	0.0	0.9	0.321097	199498	200737	1787-1787	0.500000	57.4
orf88-orf89_p4	orf88-orf89	4	ACGTCTTTCCGTTTGGACTT	TGCACCTTTTTCATCCTGCT	20	20	45.0	45.0	57.7	58.0	0.0	0.0	0.321097	199498	200737	1790-1790	0.500000	57.4
orf88-orf89_p5	orf88-orf89	5	ATAGCATCCAGAGGCTCGTA	GTGCACCTTTTTCATCCTGC	20	20	50.0	50.0	57.7	57.9	10.5	5.2	0.364711	199498	200737	1734-1734	0.500000	57.1
orf73-orf120_p1	orf73-orf120	1	AGCAGGATGAAAAAGGTGCA	AAGCACCCAGAGACAAGTTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027480	201244	202042	1495-1495	0.500000	59.8
orf73-orf120_p2	orf73-orf120	2	TGCAGCAGGATGAAAAAGGT	AAGCACCCAGAGACAAGTTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027480	201244	202042	1498-1498	0.500000	59.8
orf73-orf120_p3	orf73-orf120	3	GCAGGATGAAAAAGGTGCAC	AAGCACCCAGAGACAAGTTG	20	20	50.0	50.0	57.9	58.0	8.8	0.0	0.097390	201244	202042	1494-1494	0.500000	59.2
orf73-orf120_p4	orf73-orf120	4	GAGAGTGAATTGCAGCAGGA	AAGCACCCAGAGACAAGTTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.132134	201244	202042	1508-1508	0.500000	58.9
orf73-orf120_p5	orf73-orf120	5	AGAGAGTGAATTGCAGCAGG	AAGCACCCAGAGACAAGTTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.138029	201244	202042	1509-1509	0.500000	58.9
orf120_p1	orf120	1	TGGCAACTTCAGCTTTCTGT	AAGCACCCAGAGACAAGTTG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.084265	202043	202405	724-724	0.500000	59.3
orf120_p2	orf120	2	TGGCAACTTCAGCTTTCTGT	TTTTTCGAGGTGAGTGGCAA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.102699	202043	202405	1277-1277	0.500000	59.2
orf120_p3	orf120	3	TCGCCACAGAATGCACTTTA	AAGCACCCAGAGACAAGTTG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.204522	202043	202405	636-636	0.500000	58.4
orf120_p4	orf120	4	TCGCCACAGAATGCACTTTA	TTTTTCGAGGTGAGTGGCAA	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.222955	202043	202405	1189-1189	0.500000	58.2
orf120_p5	orf120	5	TGGCAACTTCAGCTTTCTGT	TTTTCGAGGTGAGTGGCAAT	20	20	45.0	45.0	57.9	57.7	0.0	0.0	0.327489	202043	202405	1276-1276	0.500000	57.4
orf110-rns_p1	orf110-rns	1	GCTGTCACCGATTTATCCGT	AGCAGAAACTTGACGAGCAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.110509	205090	205209	712-712	0.500000	59.1
orf110-rns_p2	orf110-rns	2	TGCTGTCACCGATTTATCCG	AGCAGAAACTTGACGAGCAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.111852	205090	205209	713-713	0.500000	59.1
orf110-rns_p3	orf110-rns	3	AACCAACTGCGTCTCTTCAG	AGCAGAAACTTGACGAGCAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.175361	205090	205209	621-621	0.500000	58.6
orf110-rns_p4	orf110-rns	4	GCTGTCACCGATTTATCCGT	ACAGACTTTCGCTTGCATCA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.181155	205090	205209	567-567	0.500000	58.6
orf110-rns_p5	orf110-rns	5	TGCTGTCACCGATTTATCCG	ACAGACTTTCGCTTGCATCA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.182499	205090	205209	568-568	0.500000	58.5