Hotspot

ycf1

SSC · 108238-113274 · 5037 bp

Back to Melanoseris

Pi 0.00322

MarkerSeek score 53.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5037
variable_sites: 37
pi: 0.00322
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTAGAATGCCGTTCCCCATT	AAGCGGGGAATGAGGATTTC	50.0	50.0	57.9	57.9	1247-1340 bp	1.0	80.2
ycf1_p2	GTAGAATGCCGTTCCCCATT	ACGGATCGACGAAAATCTGG	50.0	50.0	57.9	58.1	1168-1261 bp	1.0	80.1
ycf1_p3	GTAGAATGCCGTTCCCCATT	CGGATCGACGAAAATCTGGT	50.0	50.0	57.9	58.1	1167-1260 bp	1.0	80.1
ycf1_p4	GTAGAATGCCGTTCCCCATT	TATGACCATCGAGGGGGTTT	50.0	50.0	57.9	58.1	925-1012 bp	1.0	80.0
ycf1_p5	TGACTGGGATGTATCGTGGA	AAGCGGGGAATGAGGATTTC	50.0	50.0	57.8	57.9	650-722 bp	1.0	79.7