Hotspot

rpl20

LSC · 68056-68436 · 381 bp

Back to Melanoseris

Pi 0.00105
MarkerSeek score 30.80
Primer available yes
Label rpl20

Feature Metadata

label_name
rpl20
feature_type
gene
region
LSC
length_bp
381
variable_sites
1
pi
0.00105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20_p1 CCTTCCCGGAGTTCATTCTC ACTAGGAAAGGGGCAAAAGA 55.0 45.0 57.7 56.3 487 bp 1.0 64.3
rpl20_p2 CCTTCCCGGAGTTCATTCTC ACTAGGAAAGGGGCAAAAGAA 55.0 42.9 57.7 57.1 487 bp 1.0 62.5
rpl20_p3 GAGTTCATTCTCCGGGCAAC ACTAGGAAAGGGGCAAAAGA 55.0 45.0 58.9 56.3 479 bp 1.0 59.5
rpl20_p4 GTTCATTCTCCGGGCAACTC ACTAGGAAAGGGGCAAAAGA 55.0 45.0 58.9 56.3 477 bp 1.0 59.5
rpl20_p5 CCTTCCCGGAGTTCATTCTC ACTAGGAAAGGGGCAAAAGAAT 55.0 40.9 57.7 57.6 487 bp 1.0 58.4