Hotspot

rpl32-ndhF

SSC · 123933-124997 · 1065 bp

Back to Melanoseris

Pi 0.00188

MarkerSeek score 58.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1065
variable_sites: 5
pi: 0.00188
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1238-1259 bp	1.0	77.6
rpl32-ndhF_p2	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1236-1257 bp	1.0	77.5
rpl32-ndhF_p3	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1237-1258 bp	1.0	77.5
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1255-1276 bp	1.0	77.4
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.6	58.0	1253-1274 bp	1.0	77.3