Hotspot

ycf1-rps15

SSC · 113275-113668 · 394 bp

Back to Melanoseris

Pi 0.00558

MarkerSeek score 45.50

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 5
pi: 0.00558
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AATAATGGGAGCCGCTTGAG	CCGCAGACCTCTCTGAGATA	50.0	55.0	58.0	58.0	610 bp	1.0	80.4
ycf1-rps15_p2	AATAATGGGAGCCGCTTGAG	TCTTGTAGCTGGTCCAGTCA	50.0	50.0	58.0	58.0	832 bp	1.0	80.3
ycf1-rps15_p3	TAATTATGGAACCTGCCCGC	TCTTGTAGCTGGTCCAGTCA	50.0	50.0	58.0	58.0	1263 bp	1.0	80.2
ycf1-rps15_p4	TAATTATGGAACCTGCCCGC	CCGCAGACCTCTCTGAGATA	50.0	55.0	58.0	58.0	1041 bp	1.0	80.1
ycf1-rps15_p5	TAATTATGGAACCTGCCCGC	AGCTGGTCCAGTCATAGGTT	50.0	50.0	58.0	58.0	1257 bp	1.0	79.9