Hotspot

rpl16-rps3

LSC · 81101-82281 · 1181 bp

Back to Melanoseris

Pi 0.00305
MarkerSeek score 46.40
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1181
variable_sites
7
pi
0.00305
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AGCATATCGGCGATTGCTAG AGTAATGGGGGAACATGGGA 50.0 50.0 58.0 58.0 2479-2492 bp 1.0 80.3
rpl16-rps3_p2 CATATCGGCGATTGCTAGCT AGTAATGGGGGAACATGGGA 50.0 50.0 58.0 58.0 2477-2490 bp 1.0 80.3
rpl16-rps3_p3 GAGCATATCGGCGATTGCTA AGTAATGGGGGAACATGGGA 50.0 50.0 58.0 58.0 2480-2493 bp 1.0 80.3
rpl16-rps3_p4 GCGGGTCTTACTGTAACTGG AGTAATGGGGGAACATGGGA 55.0 50.0 58.0 58.0 2091-2103 bp 1.0 80.3
rpl16-rps3_p5 CAAGTGCCTGAAGAGCGTAT AGTAATGGGGGAACATGGGA 50.0 50.0 58.0 58.0 1967-1979 bp 1.0 80.2