Hotspot

accD-psaI

LSC · 58118-58844 · 727 bp

Back to Melanoseris

Pi 0.0033

MarkerSeek score 50.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 6
pi: 0.0033
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACACACTTTCTTTCCTTTGAATCA	AAAGAAGCCATTGCGATTGC	33.3	45.0	57.2	58.0	823-835 bp	1.0	43.5
accD-psaI_p2	ACACACTTTCTTTCCTTTGAATCA	TTGCGATTGCCGGAAATACT	33.3	45.0	57.2	58.3	813-825 bp	1.0	41.8
accD-psaI_p3	ACACACTTTCTTTCCTTTGAATCA	GCCGGAAATACTAGGCCTAC	33.3	55.0	57.2	57.5	805-817 bp	1.0	41.8
accD-psaI_p4	ACACACTTTCTTTCCTTTGA	AAAGAAGCCATTGCGATTGC	35.0	45.0	52.8	58.0	823-835 bp	1.0	41.7
accD-psaI_p5	ACACACTTTCTTTCCTTTGAATCAA	AAAGAAGCCATTGCGATTGC	32.0	45.0	57.8	58.0	823-835 bp	1.0	41.7