Hotspot

atpB-rbcL

LSC · 53914-54685 · 772 bp

Back to Melanoseris

Pi 0.00155

MarkerSeek score 53.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 3
pi: 0.00155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	927-933 bp	1.0	73.0
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	926-932 bp	1.0	72.7
atpB-rbcL_p3	CGCCCCAGTTTCTTTTTGTC	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	880-886 bp	1.0	72.2
atpB-rbcL_p4	CGCCCCAGTTTCTTTTTGTC	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	879-885 bp	1.0	72.0
atpB-rbcL_p5	TGGTAACCCCAGAACCAGAA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	855-861 bp	0.6	55.3