Hotspot

trnE-UUC-rpoB

LSC · 11968-12862 · 895 bp

Back to Melanoseris

Pi 0.00291
MarkerSeek score 53.90
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
895
variable_sites
6
pi
0.00291
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GGGACTTGAACCCTCGTTTT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.0 56.8 1034-1040 bp 1.0 62.9
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCTGA 55.0 42.9 58.1 56.8 993-999 bp 1.0 62.2
trnE-UUC-rpoB_p3 CCCTCGTTTTCTCCGTGAAA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1024-1030 bp 1.0 62.1
trnE-UUC-rpoB_p4 TTGAACCCTCGTTTTCTCCG AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1029-1035 bp 1.0 62.1
trnE-UUC-rpoB_p5 GGGACTTGAACCCTCGTTTT TCAAACCCTGATCAATGAACCT 50.0 40.9 58.0 57.6 1024-1030 bp 1.0 61.5