Hotspot

petN-psbM

LSC · 10281-10753 · 473 bp

Back to Melanoseris

Pi 0.00592

MarkerSeek score 60.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 7
pi: 0.00592
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.2	55.6	635 bp	1.0	60.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.2	56.4	635 bp	1.0	58.6
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	50.0	40.0	57.1	55.6	636 bp	1.0	54.4
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.9	55.6	627 bp	1.0	54.3
petN-psbM_p5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	47.6	40.0	58.0	55.6	621 bp	1.0	53.5