Hotspot

trnK-UUU-rps16

LSC · 4351-5117 · 767 bp

Back to Melanoseris

Pi 0.00183

MarkerSeek score 52.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 3
pi: 0.00183
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	886-916 bp	1.0	77.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	885-915 bp	1.0	77.9
trnK-UUU-rps16_p3	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	901-931 bp	1.0	77.1
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	895-925 bp	1.0	77.0
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.5	58.3	872-902 bp	1.0	75.6