Hotspot

ycf1

SSC · 125563-131010 · 5448 bp

Back to Melanosciadium

Pi 0.00593

MarkerSeek score 42.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5448
variable_sites: 48
pi: 0.00593
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTTTCTTGGCGTTGCTGTT	GCTGAGGACTTCGATGAGTG	45.0	55.0	57.8	58.1	710 bp	1.0	79.7
ycf1_p2	CTTTTCTTGGCGTTGCTGTT	TATGCGTGTTCAAAGGCGTA	45.0	45.0	57.8	57.9	1107 bp	1.0	79.3
ycf1_p3	CTTTTCTTGGCGTTGCTGTT	TATCTCAACCCCCTGAGTGG	45.0	55.0	57.8	58.1	730 bp	1.0	79.3
ycf1_p4	CTTTTCTTGGCGTTGCTGTT	GCGTGTTCAAAGGCGTAAAA	45.0	45.0	57.8	57.9	1104 bp	1.0	79.1
ycf1_p5	CTTTTCTTGGCGTTGCTGTT	ACGTGGATAAAAATCGCGGA	45.0	45.0	57.8	58.0	875 bp	0.333333	51.6