Hotspot

rpoC1

LSC · 22098-24917 · 2820 bp

Back to Melanosciadium

Pi 0.00319

MarkerSeek score 50.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2820
variable_sites: 13
pi: 0.00319
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CCGTCCTAGTTGAATCGGAG	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.5	58.0	2987-2989 bp	1.0	76.2
rpoC1_p2	CCGTCCTAGTTGAATCGGAG	ACGATCTTTGGCTCTGGAAC	55.0	50.0	57.5	57.9	2970-2972 bp	1.0	75.7
rpoC1_p3	CCGTCCTAGTTGAATCGGAG	TTCGATTGCTCGTTCGAGAA	55.0	45.0	57.5	57.7	2992-2994 bp	1.0	73.8
rpoC1_p4	CCGTCCTAGTTGAATCGGAG	TTTCGATTGCTCGTTCGAGA	55.0	45.0	57.5	57.7	2993-2995 bp	1.0	73.8
rpoC1_p5	CCGTCCTAGTTGAATCGGAG	CGATTGCTCGTTCGAGAACT	55.0	50.0	57.5	58.4	2990-2992 bp	1.0	73.5