Hotspot

ndhF

SSC · 111603-113831 · 2229 bp

Back to Melanosciadium

Pi 0.00449

MarkerSeek score 52.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 15
pi: 0.00449
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTCATGAAGGCCTACCGTTG	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.3	58.3	2344-2394 bp	1.0	76.3
ndhF_p2	AATTGGAATTCCTGTCGGGC	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2368-2418 bp	1.0	74.8
ndhF_p3	ATGAAGGCCTACCGTTGGAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.8	58.3	2341-2391 bp	1.0	72.7
ndhF_p4	CTCATGAAGGCCTACCGTTG	GGGTAGGATCCGGTAAATCG	55.0	55.0	58.3	57.3	2342-2392 bp	1.0	72.5
ndhF_p5	CTCATGAAGGCCTACCGTTG	TTGAAAGGGGGTAGGATCCG	55.0	55.0	58.3	58.8	2350-2400 bp	1.0	72.0