Hotspot

ndhF-rpl32

SSC · 113832-114855 · 1024 bp

Back to Melanosciadium

Pi 0.0104

MarkerSeek score 52.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1024
variable_sites: 16
pi: 0.0104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCGGGTCGATCAAGTAAC	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1733-1765 bp	1.0	80.9
ndhF-rpl32_p2	GTTGCCGACGGAAAAAGAAG	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1555-1587 bp	1.0	80.7
ndhF-rpl32_p3	CCGTGTGAACCAAAACCCTA	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1961-1993 bp	1.0	80.6
ndhF-rpl32_p4	TGCCGACGGAAAAAGAAGAA	TGACAAACAGGTTGATGGGG	45.0	50.0	58.0	58.0	1553-1585 bp	1.0	80.6
ndhF-rpl32_p5	TTGCCGACGGAAAAAGAAGA	TGACAAACAGGTTGATGGGG	45.0	50.0	58.0	58.0	1554-1586 bp	1.0	80.6