Hotspot

psbE-petL

LSC · 66423-67427 · 1005 bp

Back to Melanosciadium

Pi 0.00796

MarkerSeek score 66.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 10
pi: 0.00796
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GGTTACCGTTAAAGCCGCTA	45.0	50.0	58.0	58.0	893-1174 bp	1.0	84.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	882-1163 bp	1.0	84.3
psbE-petL_p3	TGACAAATAACCAACCCGCA	TGAGGTTACCGTTAAAGCCG	45.0	50.0	58.0	57.9	896-1177 bp	1.0	83.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	885-1166 bp	1.0	83.7
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.8	58.0	805-1086 bp	1.0	83.5